Electron Microscopy Sciences 63702-B10

TruBond™ Adhesion Slides 200 User Manual

Brand: Electron Microscopy Sciences

Model: 63702-B10

1. Inleiding

The TruBond™ Adhesion Slides 200 are high-quality microscope slides designed for superior sample adhesion. These slides are engineered to provide reliable performance even under challenging conditions, including high temperatures and varying pH levels, ensuring secure attachment of biological samples for microscopic examination. Each case contains 1000 blue-tinted slides, suitable for a wide range of laboratory applications.

TruBond Adhesion Slides packaging and individual slides

This image displays several boxes of TruBond Adhesion Slides, along with individual slides. The slides are designed for microscopy applications, featuring a blue tint and adhesive properties for secure sample attachment.

2. Opzetten en voorbereiden

Proper handling and preparation are crucial for optimal performance of TruBond™ Adhesion Slides. Follow these guidelines:

  1. Uitpakken: Carefully remove the slides from their packaging. Avoid touching the slide surface where the sample will be applied to prevent contamination.
  2. Opslag: Store unused slides in their original packaging in a clean, dry, and dust-free environment at room temperature to maintain their adhesive properties.
  3. Oppervlaktereiniging: While TruBond™ slides are pre-cleaned, ensure the working surface is clean and free of dust or debris before placing slides.
  4. Temperatuuracclimatisatie: Allow slides to acclimate to room temperature if they have been stored in a cooler environment, to prevent condensation which can affect adhesion.

3. Gebruiksaanwijzing

TruBond™ Adhesion Slides are designed for straightforward use in various microscopic applications. The adhesive surface ensures samples remain securely attached during staining, washing, and observation processes.

  1. Sample Toepassing: Apply the biological sample (e.g., tissue section, cell suspension) directly onto the designated adhesive area of the slide. Ensure even distribution for optimal results.
  2. Drogen: Laat de sample to dry completely on the slide. The drying time may vary depending on the sample type and environmental conditions.
  3. Staining Procedures: Proceed with standard staining protocols. The adhesive properties of the slides are designed to withstand common laboratory reagents and washing steps without sample detachment.
  4. Montage: Once staining is complete, apply a coverslip using an appropriate mounting medium.
  5. Microscopic Observation: The prepared slides are now ready for observation under a microscope.

Opmerking: For specific protocols related to your sample type, consult relevant laboratory guidelines or research papers.

4. Onderhoud en opslag

TruBond™ Adhesion Slides are single-use items and do not require maintenance after use. Proper storage before use is essential to preserve their quality.

  • Pre-Use Storage: Keep slides in their original sealed packaging until ready for use. Store in a cool, dry place, away from direct sunlight and extreme temperatures.
  • Post-Use Disposal: Dispose of used slides according to your laboratory's biohazard waste disposal protocols. Do not reuse slides.
  • Besmettingspreventie: Always handle slides by the edges to prevent fingerprints or other contaminants from affecting the adhesive surface.

5. Probleemoplossing

While TruBond™ Adhesion Slides are designed for reliability, occasional issues may arise. Here are some common problems and their potential solutions:

ProbleemMogelijke oorzaakOplossing
Poor Sample Adhesion
  • Contaminated slide surface (e.g., fingerprints, dust).
  • Ongepaste sample preparation or drying.
  • Expired or improperly stored slides.
  • Handle slides by edges only. Ensure clean working environment.
  • Verify sample preparation protocols. Ensure adequate drying time.
  • Check slide expiration date. Store slides as recommended.
Bubbles under Coverslip
  • Too much or too little mounting medium.
  • Improper coverslip application.
  • Use the correct amount of mounting medium.
  • Apply coverslip slowly from one edge to the other to push out air.
Staining Irregularities
  • Inconsistente sample applicatie.
  • Issues with staining reagents.
  • Ensure even spreading of sample on the slide.
  • Verify reagent quality and protocol adherence.

6. Specificaties

AttribuutDetail
ProductnaamTruBond™ Adhesion Slides 200
Modelnummer63702-B10
MerkElektronenmicroscopie Sciences
Aantal per doos1000 dia's
KleurBlauw
Belangrijkste kenmerkProven performance under high temperature and pH conditions
ASINB07F2S748R
Datum eerste beschikbaarheid2 mei 2014

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